CRISPR/Cas9技术的应用性研究

doi:10.11843/j.issn.0366-6964.2016.07.003

Abstract

Abstract:

To investigate new application of CRISPR/Cas9 technology，we established a method to study genes function in living organism by the application of CRISPR/Cas9 in genetic manipulation.By using in vivo electroporation，immunohistochemistry and PCR analyzing，we first validated the function of DCX and the results indicated that CRISPR/Cas9 could be used in down-regulated gene expression in mouse genetic manipulation.Then this method was also used to study the function of constant exons of Pcdhα cluster. To further clarify the mechanism that led to the phenotype of neuron migration defects by DCX，we designed experiments to identify the genotype of target genes.The results indicated that CRISPR/Cas9 could lead to deletion，inversion and duplication of target genes.In conclusion，CRISPR/Cas9 technology could be used in mouse genetic manipulation and edit the target genes efficiently，which might be an ideal method to research gene function in living organism.

CLC Number:

S813.3

SHU Lei-lei，JIA Zhi-lian，WU Yong-hu，SUO Lun，JIA Li-ling. The Application Research of CRISPR/Cas9[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1316-1323.

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The Application Research of CRISPR/Cas9

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